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Description
Hello, trying the tool for the first time.
@OHRI-bell-virt ~/programs/nanocorrect $ make -f nanocorrect-overlap.make INPUT=/scratch/apelin/MinION_sequencing/Kevin_plasmid/MinION/Pass_2D_seqtk.fasta NAME=nc
nanocorrect-preprocess.pl /scratch/apelin/MinION_sequencing/Kevin_plasmid/MinION/Pass_2D_seqtk.fasta > nc.pp.fasta
fasta2DB nc nc.pp.fasta
DBsplit -s50 nc
DBdust nc
HPCdaligner -t5 -mdust nc > HPCcommands.txt
/bin/bash HPCcommands.txt
LAcat nc > nc.las
rm nc.*.las
So far so good.
Then got pip to install pysam and biopython.
Second step:
@OHRI-bell-virt ~/programs/nanocorrect $ python nanocorrect.py nc all > corrected.fasta
[fai_load] build FASTA index.
Traceback (most recent call last):
File "nanocorrect.py", line 183, in
(start, end) = [ int(x) for x in read_range.split(':') ]
ValueError: invalid literal for int() with base 10: 'all'
Not sure what is causing this.
@OHRI-bell-virt ~/programs/nanocorrect $ python -V
Python 2.7.9
Adrian