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README.md
@@ -36,6 +36,9 @@ We evaluated seven normalization approaches for all methods:
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6. [Training Distribution Matching](https://peerj.com/articles/1621/) (TDM)
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7. z-scoring (Z)
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+We also explored the use of [Seurat](https://satijalab.org/seurat/) to normalize array and RNA-seq data.
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+Due to low sample numbers at the edges of our titration protocol, many experimental conditions could not be integrated.
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+
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## Requirements
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We recommend using the docker image `envest/rnaseq_titration_results:R-4.1.2` to handle package and dependency installation.
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