diff --git a/_pydev_bundle/pydev_is_thread_alive.py b/_pydev_bundle/pydev_is_thread_alive.py
index 3f2483a1..e2887f51 100644
--- a/_pydev_bundle/pydev_is_thread_alive.py
+++ b/_pydev_bundle/pydev_is_thread_alive.py
@@ -5,7 +5,12 @@
 # It is required to debug threads started by start_new_thread in Python 3.4
 _temp = threading.Thread()
 
-if hasattr(_temp, "_handle") and hasattr(_temp, "_started"):  # Python 3.13 and later has this
+if hasattr(_temp, "_os_thread_handle") and hasattr(_temp, "_started"):  # Python 3.14 has no `_handle`
+
+    def is_thread_alive(t):
+        return not t._os_thread_handle.is_done()
+
+elif hasattr(_temp, "_handle") and hasattr(_temp, "_started"):  # Python 3.13 and later has this
 
     def is_thread_alive(t):
         return not t._handle.is_done()
diff --git a/_pydevd_sys_monitoring/_pydevd_sys_monitoring.py b/_pydevd_sys_monitoring/_pydevd_sys_monitoring.py
index 5d97a67d..00c2c32c 100644
--- a/_pydevd_sys_monitoring/_pydevd_sys_monitoring.py
+++ b/_pydevd_sys_monitoring/_pydevd_sys_monitoring.py
@@ -255,7 +255,6 @@ def _get_unhandled_exception_frame(exc, depth: int) -> Optional[FrameType]:
 #     cdef PyDBAdditionalThreadInfo additional_info
 #     thread: threading.Thread
 #     trace: bool
-#     _use_is_stopped: bool
 # ELSE
 class ThreadInfo:
     additional_info: PyDBAdditionalThreadInfo
@@ -276,8 +275,11 @@ def __init__(self, thread: threading.Thread, thread_ident: int, trace: bool, add
         self.thread_ident = thread_ident
         self.additional_info = additional_info
         self.trace = trace
-        self._use_is_stopped = hasattr(thread, '_is_stopped')
-        
+
+        self._use_handle = hasattr(thread, "_handle")
+        self._use_started = hasattr(thread, "_started")
+        self._use_os_thread_handle = hasattr(thread, "_os_thread_handle")
+
     # fmt: off
     # IFDEF CYTHON
     # cdef bint is_thread_alive(self):
@@ -285,11 +287,18 @@ def __init__(self, thread: threading.Thread, thread_ident: int, trace: bool, add
     def is_thread_alive(self):
     # ENDIF
     # fmt: on
-        if self._use_is_stopped:
-            return not self.thread._is_stopped
-        else:
+        # Python 3.14
+        if self._use_os_thread_handle and self._use_started:
+            return not self.thread._os_thread_handle.is_done()
+
+        # Python 3.13
+        elif self._use_handle and self._use_started:
             return not self.thread._handle.is_done()
 
+        # Python 3.12
+        else:
+            return not self.thread._is_stopped
+
 
 class _DeleteDummyThreadOnDel:
     """
diff --git a/_pydevd_sys_monitoring/_pydevd_sys_monitoring_cython.pyx b/_pydevd_sys_monitoring/_pydevd_sys_monitoring_cython.pyx
index 94e70e71..1afae88a 100644
--- a/_pydevd_sys_monitoring/_pydevd_sys_monitoring_cython.pyx
+++ b/_pydevd_sys_monitoring/_pydevd_sys_monitoring_cython.pyx
@@ -247,7 +247,6 @@ cdef class ThreadInfo:
     cdef PyDBAdditionalThreadInfo additional_info
     thread: threading.Thread
     trace: bool
-    _use_is_stopped: bool
 # ELSE
 # class ThreadInfo:
 #     additional_info: PyDBAdditionalThreadInfo
@@ -268,8 +267,11 @@ cdef class ThreadInfo:
         self.thread_ident = thread_ident
         self.additional_info = additional_info
         self.trace = trace
-        self._use_is_stopped = hasattr(thread, '_is_stopped')
-        
+
+        self._use_handle = hasattr(thread, "_handle")
+        self._use_started = hasattr(thread, "_started")
+        self._use_os_thread_handle = hasattr(thread, "_os_thread_handle")
+
     # fmt: off
     # IFDEF CYTHON -- DONT EDIT THIS FILE (it is automatically generated)
     cdef bint is_thread_alive(self):
@@ -277,11 +279,18 @@ cdef class ThreadInfo:
 #     def is_thread_alive(self):
     # ENDIF
     # fmt: on
-        if self._use_is_stopped:
-            return not self.thread._is_stopped
-        else:
+        # Python 3.14
+        if self._use_os_thread_handle and self._use_started:
+            return not self.thread._os_thread_handle.is_done()
+
+        # Python 3.13
+        elif self._use_handle and self._use_started:
             return not self.thread._handle.is_done()
 
+        # Python 3.12
+        else:
+            return not self.thread._is_stopped
+
 
 class _DeleteDummyThreadOnDel:
     """