Skip to content

MQ could not identify modified peptides when using localized enzyme mode #175

@A-dealbata

Description

@A-dealbata

Describe the bug
In our MaxQuant (v.2.6.0), when we set a localized enzyme mode (e.g. elastase at the C-terminal of AGILSV, specific or semi-specific), the identified peptides were all unmodified. All other parameters were the default.

To Reproduce
Steps to reproduce the behavior:

  1. Go to set a localized enzyme: elastase at the C-terminal of AGILSV (or any other localized enzyme mode)
  2. Select specific or semi-specific digestion
  3. using variable modification deamination NQ and Oxidation M (or any other modifications).
  4. Run MQ with any raw data and database
  5. See error in the output .txt docs

Expected behavior
Identify both modified and unmodified peptides.

Screenshots

Desktop (please complete the following information):

  • OS: Ubuntu
  • Version 20.04

Additional context
Using a higher version of MQ could not solve this problem (in my test).

Metadata

Metadata

Assignees

No one assigned

    Labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions