-
Notifications
You must be signed in to change notification settings - Fork 7
Open
Description
Currently we use this kind of syntax to specify the genome and primer scheme:
--genome 'MN908947.3' \
--primer_set artic \
--primer_set_version 3
This is equivalent to (links from artic repo):
--fasta https://raw.githubusercontent.com/artic-network/artic-ncov2019/master/primer_schemes/nCoV-2019/V3/nCoV-2019.reference.fasta \
--bed https://raw.githubusercontent.com/artic-network/artic-ncov2019/master/primer_schemes/nCoV-2019/V3/nCoV-2019.primer.bed
Advantages of the latter syntax:
- Can also be used using local files (i.e. the files can be downloaded beforehand).
- Allows using other reference genomes (e.g. other viruses) or custom primer schemes.
- Doesn't depend on
viralrecon
developers to have made the primer scheme you are using available. For example, do you want to use NEB VARSkip primer set "2b"? You can get them from their repo and then specify:
--fasta https://raw.githubusercontent.com/nebiolabs/VarSkip/main/schemes/NEB_VarSkip/V2b/NEB_VarSkip.reference.fasta \
--bed https://raw.githubusercontent.com/nebiolabs/VarSkip/main/schemes/NEB_VarSkip/V2b/NEB_VarSkip.scheme.bed
Metadata
Metadata
Assignees
Labels
No labels