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Main parameters

Parameter	Description	Situations	Recommended
Pixel size (nm)	For iterative deconvolution	----	Fit your system
Wave length (nm)	For iterative deconvolution	----	Fit your system
Numerical aperture	For iterative deconvolution	----	Fit your system, and algorithm parameters
Sparse iteration times	Sparsity recon. iteration times	Over-sampling (Spatial on/ off)	300/100
Image fidelity	Data fidelity	Over-sampling (Spatial on/off)	low/high
t(z)axial continuity	Data continuity in t or z	-----	0~1
Sparsity	Data sparsity	Artifacts little/much	low/high
Iterative deblur times	The iteration times of deblur	-----	5-15(LR)/30-50(LW)

Options:

• 3D imaging: The input images are whether or not 3D images.
• GAIN: the brightness of the image can be altered via this control bar.
• Background: The background estimation level chosen by user.
• Iterative deconvolution : The deconvolution method chosen by user.
• Up-sampling: The up-sampling method chosen by user.