Quant - LFQ - DDA - Astral instrument

[brvpuyve]

aim of the new module

New module proposed by C4L on DDA data generated on a Thermo Astral.
Would be an additional module next to the Quant - DDA - Orbitrap QE HF-X precursor/peptidoform level, yet with a different instrument design (Orbitrap-TOF).

full description of the new module

1. What file would be available for download on the module website?
Data is not yet public, but will be in the upcoming months.
Currently the data can be downloaded from: https://filesender.belnet.be/?s=download&token=7b7d5b37-a154-446b-9db3-ac5dffae0af5
Data file names:
LFQ_Astral_DDA_15min_50ng_Condition_A_REP1.raw
LFQ_Astral_DDA_15min_50ng_Condition_A_REP2.raw
LFQ_Astral_DDA_15min_50ng_Condition_A_REP3.raw
LFQ_Astral_DDA_15min_50ng_Condition_B_REP1.raw
LFQ_Astral_DDA_15min_50ng_Condition_B_REP2.raw
LFQ_Astral_DDA_15min_50ng_Condition_B_REP3.raw

2. What would users need to do?
Users need to download the data and run it through typical DDA workflows. C4L has already started generating output.

3. What would be the type/format of files used as input for the module?
See Quant-DDA module
4. What metrics would be calculated and how?
See Quant-DDA module
5. Propose a plot for metrics visualisation
See Quant-DDA module

potential reviewers

NA

Will you be able to work on the implementation (coding) yourself, with additional help from the ProteoBench maintainers?

• yes
• no

any other information

NA

[brvpuyve]

DDA module for an Astral dataset (3x Condition A and 3x Condition B) was added: https://proteobench.cubimed.rub.de/Quant_LFQ_DDA_ion_Astral

The documentation is ready, but there still seems to be an issue with the redirection of the link (issue was opened).
The data is also available to the public now (from the Bochum servers). Data will be uploaded to ProteomeXchange in the upcoming months.