-
Notifications
You must be signed in to change notification settings - Fork 115
Description
I trained augustus and tried running on my masked FASTA file for the gene prediction but it is showing me this error of invalid nucleotide encountered.
s2769179@egadmin-HP-EliteDesk-800-G3-TWR:$ source $ augustus --species=glycinemax "/home/s2769179/ncbi-cxx-toolkit-public-release-29.0.0/src/app/winmasker/MC50_masked.fasta" > MC50_output.gff/conda/bin/activate$ conda activate augustus
(base) s2769179@egadmin-HP-EliteDesk-800-G3-TWR:
(augustus) s2769179@egadmin-HP-EliteDesk-800-G3-TWR:
augustus: ERROR
Invalid nucleotide '' encountered.
(augustus) s2769179@egadmin-HP-EliteDesk-800-G3-TWR:~$ grep "" /home/s2769179/ncbi-cxx-toolkit-public-release-29.0.0/src/app/winmasker/MC50_masked.fasta
^C
(augustus) s2769179@egadmin-HP-EliteDesk-800-G3-TWR:~$ grep "\" /home/s2769179/ncbi-cxx-toolkit-public-release-29.0.0/src/app/winmasker/MC50_masked.fasta
grep: Trailing backslash
If anyone knows how this error could be troubleshooted, kindly let me know.